Figure 1.
FRET biosensors recapitulate Ran-regulated association of XCTK2 and importin α/β. (A–C) Schematic (left) and solution-based FRET assay (right) of importin α-CyPet with YPet-XCTK2 ± importin β (A) ± RanQ69L (B) or YPet-XCTK2-ΔNLS + importin β (C). The normalized FRET ratios are graphed as the mean ± SEM from 440 to 600 nm (n = 3 independent experiments).

FRET biosensors recapitulate Ran-regulated association of XCTK2 and importin α/β. (A–C) Schematic (left) and solution-based FRET assay (right) of importin α-CyPet with YPet-XCTK2 ± importin β (A) ± RanQ69L (B) or YPet-XCTK2-ΔNLS + importin β (C). The normalized FRET ratios are graphed as the mean ± SEM from 440 to 600 nm (n = 3 independent experiments).

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