Figure 4.
KIF4A FF1154 fails to support radial compaction of the anaphase central spindle.(A) Superresolution images of KIF4A WT and the FF1154AA mutant, showing anaphase localization of KIF4A and PRC1 before furrowing (6–8 min) and during furrowing (14–16 min). Stacks were acquired to capture the total volume on the cell and the radial distribution of PRC1 at the cell equator is showed in the 90° rotation. (B) Superresolution images of WT KIF4A and the FF1154AA mutant showing the distribution of KIF4A and MKLP1 in anaphase before furrowing (6–8 min) and during furrowing (14–16 min). Stacks were acquired to capture the total volume on the cell and the radial distribution of MKLP1 at the cell equator is showed in the 90° rotation. The white arrows mark the edge of the cell, and the pink arrows mark the bundles of PRC1 and MKLP1. Cartoons on the right depict PRC1 and MKLP1 distribution in WT and FF1154AA cells.

KIF4A FF1154 fails to support radial compaction of the anaphase central spindle. (A) Superresolution images of KIF4A WT and the FF1154AA mutant, showing anaphase localization of KIF4A and PRC1 before furrowing (6–8 min) and during furrowing (14–16 min). Stacks were acquired to capture the total volume on the cell and the radial distribution of PRC1 at the cell equator is showed in the 90° rotation. (B) Superresolution images of WT KIF4A and the FF1154AA mutant showing the distribution of KIF4A and MKLP1 in anaphase before furrowing (6–8 min) and during furrowing (14–16 min). Stacks were acquired to capture the total volume on the cell and the radial distribution of MKLP1 at the cell equator is showed in the 90° rotation. The white arrows mark the edge of the cell, and the pink arrows mark the bundles of PRC1 and MKLP1. Cartoons on the right depict PRC1 and MKLP1 distribution in WT and FF1154AA cells.

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