Figure 4.
Facilitated transport of MS2 capsids into FG-Nup49 droplets. Epifluorescence microscopy of whole device. Panels are organized analogously to Fig. 2. (A) Interaction between the MS2-NLS capsid and the FG-Nup49 droplets in the presence of Importin α/Importin β, showing the cargo entering into the droplets (see also zoom). (B) The negative experiment: MS2 capsid without NLSs. For all rows: FG-Nups49 droplet channel is displayed in red, MS2 cargo channel is in grayscale, and the ratiometric projection of the full experiment shows colorimetric interaction. Scale bar is 50 µm. Shown are exemplary datasets from two technical replicates.