Passive exclusion properties of the liquid state of FG-Nup49. Confocal microscopy of flowing droplets. (A–C) The passive exclusion of the three cargoes from the FG-Nup49 droplets. The first row (A) shows confocal images in permeabilized cell experiments analogous to Fig. 2. The confocal images below (B) show the FG-Nup49 droplets represented in red and the cargo in grayscale. The last row shows the line profiles of the three droplets in the mCherry experiment (C) along the orange lines. All experiments were performed in the absence of NTRs. Fig. S3 shows a validation that an IBB-mCherry cargo in the presence of Importin β is imported. Scale bar is 20 µm. See corresponding Videos 4, 5, and 6.