Epifluorescence whole device/chip pipeline analysis. This figure summarizes the analysis pipeline to calculate cargo enrichment into the droplets when imaging the whole device using a 10× objective and epifluorescence microscopy. (A) Red signal from fluorescence of FG-Nup49 droplets. (B) Mask obtained by segmenting the red channel (FG-Nup49). (C) Green signal from the cargo fluorescence. (D) Signal of the cargo only in the region of interest (droplets region) obtained by applying mask B to image in C. (E) Colorimetric image of the ratio between the sum of A and D divided by D. (F) Maximum-intensity projection of the full ratio video. Scale bar is 50 µm.