Mislocalized LRRK2 phosphorylates and traps pRab10. (A) Light microscopy of an A549 Rab10 knockout cell expressing untagged GBP, GFP-R1441G LRRK2 (green), and Myc-Rab10 (cyan). pRab10 is shown in red. (B) Light microscopy of a HeLa cell stained for expressed mito-GBP (magenta) and “mito” GFP-R1441G LRRK2 (green). Mitofilin staining shown in red. (C) Light microscopy of a HeLa cell expressing mito-GBP, GFP-R1441G LRRK2 (green), and Myc-Rab10 (red). Mitofilin is shown in cyan. (D) Light microscopy of a HeLa cell expressing mito-GBP, GFP-D2017A LRRK2 (green), and Myc-Rab10 (red). Mitofilin is shown in cyan. (E) Light microscopy of a HeLa cell expressing PM-GBP, GFP-R1441G LRRK2 (green), and Myc-Rab10 (cyan); pMyc-Rab10 shown in red. The entire Z-stack series of GFP in this cell is shown in Video 1. Scale bars, 10 µm.