Figure S3.
![Localization of talinIVVI to FAs in talinKO cells. (A) Coexpression of a GFP-talin∆FERM construct with mCh-vinFL-cBAK in NIH3T3 cells reveals this construct can bind to inactive vinculin. Scale bar indicates 10 µm. (B) Coexpression of GFP-talL897S with mCh-vinFL-cBAK in NIH3T3 cells reveals that destabilizing the R3 helical bundle through this point mutation (Rahikainen et al., 2017) permits talin–vinculin binding. Scale bar indicates 10 µm. (C) Expression of GFP-talIVVI (containing R3 stabilizing mutations [Goult et al., 2013b]) in talinKO cells shows this construct localizes to FAs.](https://cdn.rupress.org/rup/content_public/journal/jcb/219/1/10.1083_jcb.201903134/6/jcb_201903134_figs3.png?Expires=1770856208&Signature=JviOiZN-upjVyJKqU3iiAEvQqmTjqZ9ZnyDCnMfQFvSkBJ2XQ2Dwq-gmkVNfyMr0RodVqqKdAvP9j6VlsPQjwBykP4Jq12P-WCvjSMet5B7lOfwgyoJ6mI~e-kW4pKBp-6Irv4nOMXOCeXiZ54rOE45xCgypC0PFuVHMqRnX3GXp-0Oz-KJozwh6yHw070Iwpd3d9jFbyTDPmTMAwtOyczxPSXcurG1Ty4JPnWj8SBqkzqgvWdWwiYVheBm95m~JyK51vgC9GY7XytoJdt8tdp29i7hudTAFzkvRTTIjEKe0awFGOFDWQwV1d0CNUC9xynw5CSJbyMCj6Os3jhCYuA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Localization of t alinIVVI to FAs in talinKO cells. (A) Coexpression of a GFP-talin∆FERM construct with mCh-vinFL-cBAK in NIH3T3 cells reveals this construct can bind to inactive vinculin. Scale bar indicates 10 µm. (B) Coexpression of GFP-talL897S with mCh-vinFL-cBAK in NIH3T3 cells reveals that destabilizing the R3 helical bundle through this point mutation (Rahikainen et al., 2017) permits talin–vinculin binding. Scale bar indicates 10 µm. (C) Expression of GFP-talIVVI (containing R3 stabilizing mutations [Goult et al., 2013b]) in talinKO cells shows this construct localizes to FAs.
