Spatiotemporal characterization of localized dendritic Ca²⁺ transients. (A) Schematic of dendritic Ca²⁺ transient (green) spread from one origin over time (t). (B) Dendritic Ca²⁺ transient from single detectable origin site (*). (C) Ca²⁺ spread at six time points (full-width half maximum Gaussian fit as thick lines) and time courses at five distances from the initiation sight (*) in the single dendrite (B). (D) Time-lapse imaging of Ca²⁺ transients originating at two adjacent regions (* and >) in a single co-cultured melanocyte dendrite. (E) Spatiotemporal dynamics of a single representative dendritic transient. Left: Ca²⁺ spread at the eight corresponding times marked in D. Right: Time course of Ca²⁺ transients at the five indicated distances from the initiation site (*); region > is represented at −5.5 µm with solid black line. (F) Kymograph of line along the dendrite in B. Repetitive Ca²⁺ transients (white arrows) following the transient (*) in B. Scale bars, (B) 5 µm; (D) 5 µm. See also Video 2.