Figure 4.
The CHC22 compartment is localized proximal to the TGN and does not overlap with the cis-Golgi. (A–C) Representative SIM of the perinuclear region of HeLa-GLUT4 cells (HeLa-G4) and hSkMC-AB1190-GLUT4 (hSkMC) stained for CHC22 (red) and GM130 (A), TGN46 (B), and STX-6 (blue; C). The solid gray lines delineate the nuclear border (N). The dashed white lines span the segment over which fluorescence intensities for GLUT4 (green), CHC22 (red), and GM130, TGN46, and STX-6 (blue) were plotted. Scale bars: 1 µm. In A–C, GLUT4 (green) was detected by GFP tag in HeLa-GLUT4 or immunostained using an anti-GFP antibody in hSkMC-AB1190-GLUT4. Merged images show red/green overlap in yellow, red/blue overlap in magenta, green/blue overlap in cyan, and red/green/blue overlap in white.

The CHC22 compartment is localized proximal to the TGN and does not overlap with the cis-Golgi. (A–C) Representative SIM of the perinuclear region of HeLa-GLUT4 cells (HeLa-G4) and hSkMC-AB1190-GLUT4 (hSkMC) stained for CHC22 (red) and GM130 (A), TGN46 (B), and STX-6 (blue; C). The solid gray lines delineate the nuclear border (N). The dashed white lines span the segment over which fluorescence intensities for GLUT4 (green), CHC22 (red), and GM130, TGN46, and STX-6 (blue) were plotted. Scale bars: 1 µm. In A–C, GLUT4 (green) was detected by GFP tag in HeLa-GLUT4 or immunostained using an anti-GFP antibody in hSkMC-AB1190-GLUT4. Merged images show red/green overlap in yellow, red/blue overlap in magenta, green/blue overlap in cyan, and red/green/blue overlap in white.

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