Role of P/Q- and L-type calcium currents in electrophysiological abnormalities of lamin A/C haploinsufficient hiPSC-CMs. (A and B) Representative quantifications of electrophysiological properties from MEA analyses in standard culture conditions or after treatment with the indicated inhibitors for P/Q-type calcium channels (ω-conotoxin MVIIC: 2 µM; ω-agatoxin TK: 0.5 µM). Differences versus mutant were calculated by one-way ANOVA with post hoc Holm–Sidak binary comparisons (*, P < 0.05; **, P < 0.01; n = 3–8 wells; average ± SEM). (C and D) RT-qPCR validation of gene expression changes in hiPSC-CMs matured by culture in vitro for 30 d (C) or by generation of 3D-EHTs (D). Differences versus mutant were calculated by one-way ANOVA with post hoc Holm–Sidak binary comparisons (*, P < 0.05; **, P < 0.01; ***, P < 0.001; n = 4 differentiations for panel C, and n = 3 3D-EHT batches for panel D; average ± SEM). (E) As in panels A and B, but hiPSC-CMs were treated with increasing doses of the L-type calcium channel blocker verapamil (*, P < 0.05; ***, P < 0.001; n = 1–4 wells; average ± SEM).