Alterations in peripheral localization of lamin A/C–sensitive loci. (A) Representative immunoFISH for the nuclear lamina (lamin B1), the cardiac marker α-actinin, and the CACNA1C locus in mutant and corrected hiPSCs and hiPSC-CM (nuclei counterstained with DAPI). Scale bars, 5 µm. (B) Quantification of the distance between the indicated loci and the nuclear lamina in diploid cells based on immunoFISH data. Violin plots report the whole range, and horizontal lines indicate the first quartile, median, and third quartile. Statistical analysis by Brown–Forsythe and Welch ANOVA test followed by the Holm–Sidak multiple comparisons versus hiPSC for the same line or versus mutant, as indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001; n = individual loci, as indicated). (C) As in Fig. 6 E, but reporting chromatin compartmentalization changes for two genomic regions not affected by lamin A/C haploinsufficiency and used as negative control for immunoFISH experiments (B).