Global properties of chromatin topology in lamin A/C haploinsufficient hiPSC-CMs. (A) Hierarchical clustering of hiPSC-CMs analyzed by in situ DNase Hi-C based on similarity scores between the genomic contact matrices calculated with HiCRep. (B) Proportion of trans or cis interactions involving distances <20 kb (cis short) or >20 kb (cis long; Table S4). Differences versus mutant were calculated by two-way ANOVA with post hoc Holm–Sidak binary comparisons (*, P < 0.05; n = 2 differentiations; average ± SEM). (C) Representative heatmaps of differential contact matrices between autosomes (ordered by size). (D) Representative heatmaps of differential cis interactions between A and B compartments. (E) Probability of cis genomic contacts over increasing genomic distance for regions in homotypic (A-A or B-B) or heterotypic (A-B) chromatin compartments (gray background: 95% confidence bands). obs/exp, observed/expected.