Electrophysiological properties of lamin A/C haploinsufficient hiPSC-CMs. (A) Representative traces from MEA recordings of spontaneous electrical activity in hiPSC-CM monolayers. (B) Representative quantifications of electrophysiological properties from MEA analyses. Differences versus mutant were calculated by one-way ANOVA with post hoc Holm–Sidak binary comparisons (**, P < 0.01; ***, P < 0.001; n = 5–16 wells; average ± SEM). (C) Representative voltage recordings by whole-cell patch clamp during evoked action potentials in individual hiPSC-CMs. (D) Quantifications of electrophysiological properties from whole-cell patch clamp analyses (APD90, action potential duration at 90% repolarization). Differences versus mutant were calculated by one-way ANOVA with post hoc Holm–Sidak binary comparisons (*, P < 0.05; **, P < 0.01; ***, P < 0.001; n = 26–30 cells from two differentiations; average ± SEM). (E) Representative optical recordings of calcium fluxes with Fluo-4 in hiPSC-CM monolayers electrically paced at 1 Hz (Fmax, peak fluorescence; F0, baseline fluorescence). (F) Representative quantifications of calcium fluxes properties. Differences versus mutant were calculated by unpaired t test (***, P < 0.001; n = 69–70 cells; average ± SEM).