SNX3 enables recruitment of Rab5a and galectin-9 to borreliae phagosomes. (A and B) Confocal micrographs of macrophages expressing RFP-Rab5 and stained for galectin-9 using specific antibody, incubated with GFP-expressing borreliae, and treated with control siRNA (A) or SNX3-specific siRNA (B). Scale bars: 10 µm for overview images and 1 µm for detail images. (C–E) Statistical evaluation of RFP-Rab5a– (C) and galectin-9–based (D) fluorescence intensities at borreliae phagosomes in cells treated with control siRNA or SNX3-specific siRNA, with respective measured volume of confocal stacks in cubic micrometers. Values are given as mean ± SEM (n = 3 × 20). For specific values, see Table S1. Two-tailed Student’s t test; **, P < 0.01.