Evaluation of borreliae phagosome compaction in macrophages expressing siRNA-insensitive galectin-9 constructs. (A and C) Domain structure of galectin-9, showing an N-terminal extension (N) and two CRDs (CRD1 and CRD2), which are connected by a linker region (link), and respective deletion constructs (A) or point mutants defective in the binding of specific carbohydrates (see text; C). (B and D) Statistical evaluation of borreliae compaction in cells treated with control siRNA or galectin-9–specific siRNA and expressing indicated deletion (B) or point mutation (D) constructs. B: at least n = 4 (4 × 20 cells); D: n = 3 (3 × 15 cells). Dotted red line indicates level of borreliae compaction in galectin-9–depleted control (GFP-expressing) cells. For specific values, see Table S1. One-way ANOVA; *, P < 0.05; **, P < 0.01. Error bars: mean ± SEM.