HVEM examination of lysosomes in the hypodermis of N2,slc-36.1(yq110), andppk-3(yq24) adult animals. Membrane-bound spherical organelles filled with membrane debris were identified as lysosomes. (A) HVEM images of electron-dense and electron-lucent lysosomes in the hypodermis of N2 animals. Bars, 0.5 µm. (B) HVEM images (top) of lysosomes undergoing different steps of lysosomal reformation in N2 hypodermis. Traces of lysosome reformation at each step are shown underneath. Bars, 0.5 µm. (C) HVEM images of electron-lucent lysosomes in the hypodermis of slc-36.1(yq110),ppk-3(yq24), and ppk-3(n2668) animals. Bars, 0.5 µm. (D) Quantification (mean ± SEM) of lysosome sizes in N2, slc-36.1(yq110),ppk-3(yq24), and ppk-3(n2668) adult hypodermis. 50 lysosomes were measured for each genotype. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (E) Percentage of lysosomes with different morphologies shown in A–C in N2, slc-36.1(yq110), ppk-3(yq24), and ppk-3(n2668) adult hypodermis. For each genotype, indicated number of lysosomes from ≥10 electron microscopic sections of two or three worms were analyzed. (F) Graphic summary of the SLC-36.1–PPK-3 axis in lysosome reformation from phagolysosomes and autolysosomes.