PPK-3/PIKfyve is required for PLR. (A) Images of LAAT-1::GFP– and HIS-24::mCh–positive embryonic cell corpses and vacuoles in N2, ppk-3(yq24), andppk-3(n2668) comma-stage embryos. Arrows and arrowheads indicate button-like cell corpses and vacuoles, respectively. Boxed regions are magnified (2.5×) in insets. Bars, 2 µm.(B) Time-course quantification of embryonic vacuoles in the indicated strains. 15 embryos were scored at each stage for each strain. ***, P < 0.001. (C) Time-lapse chasing of a LAAT-1::GFP– and HIS-24::mCh–positive cell corpse in appk-3(n2668) embryo. The time point that the LAAT-1::GFP ring was first detected on the cell corpse was set as 0 min. The arrow indicates lysosome fusion. Bars, 2 µm. (D and E) Quantification (mean ± SEM) of lysosome tubulation (D) and change in phagolysosome sizes (E). 15 cell corpses were analyzed. **, P < 0.01; ***, P < 0.001. (F) DIC image of an N2 comma-stage embryo showing the cell corpse-engulfing ABplaapppp cell and fluorescent images of N2, slc-36.1(yq110), andppk-3(n2668) comma-stage embryos expressing LAAT-1::GFP. ABplaapppp cells are indicated by dotted circles and magnified (2.4×) in insets. Asterisks and arrowheads indicate phagolysosomes and lysosomes, respectively. Bars, 2 µm.(G) Quantification (mean ± SEM) of lysosomes in ABplaapppp cells. 40 or more ABplaapppp cells in each genotype were analyzed. ***, P < 0.001.