slc-36.1 mutant embryos contain vacuoles arising from apoptotic cell corpses. (A) DIC images of WT (N2),yq51, yq80, andyq110 embryos. Arrows and arrowheads indicate button-like cell corpses and vacuoles, respectively. Boxed regions are magnified (2.5×) in insets. Bars, 2 µm. (B) Quantification (mean ± SEM) of vacuoles in 2-fold and 4-fold embryos in N2 and mutants. 15 embryos were scored at each stage for each strain. (C) Schematic representation of theslc-36.1 gene and the encoded protein. Black boxes and thin lines indicate exons and introns, respectively. Mutation sites in all mutants are indicated. Blue boxes indicate amino acid transporter domain. (D) Quantification (mean ± SEM) of embryonic vacuoles in the indicated strains. 15 embryos at the 1.5-fold and 4-fold stages were scored for each strain. ***, P < 0.001. (E and F) Time-lapse recording of cell corpses in N2 andslc-36.1(yq110) embryos. Arrows and arrowheads indicate cell corpses and vacuoles, respectively (E). Bars, 2 µm. 33 cell corpses were examined for each strain. The duration of each stage (mean± SEM) is shown in F. (G) Quantification (mean ± SEM) of embryonic vacuoles in 1.5-fold and 4-fold embryos with the indicated genotypes. 15 embryos were scored at each stage (1.5F, 2F, 2.5F, 3F, and 4F represent 1.5-, 2-, 2.5-, 3-, and 4-fold, respectively) for each strain. ***, P < 0.001. H. sapiens, Homo sapiens.