Drg1 is required for planar polarization and apical docking of basal bodies in MCCs. (A) Planar polarization defect in Drg1 morphants. CLAMP-GFP (rootlet marker) and centrin-RFP (basal body marker) were coinjected with the mixture of the indicated MOs and mRNAs. Areas enclosed by the red box are magnified in the insets, and arrows indicate the orientation of basal body/rootlets in MCCs. The circular plots depict polarity of basal body/rootlets in MCCs. Each arrow indicates the mean polar direction of basal body/rootlets in a single MCC. Arrow length depicts 1 minus circular variance of basal body/rootlet orientation around the mean. Different colored arrows represent data from different embryos. Scale bars, 5 µm. (B) Basal body docking defect at the apical regions of MCCs upon Drg1 knockdown. CLAMP-GFP and centrin-RFP mRNAs were coinjected with the cocktail of the indicated MOs and mRNAs. Serial z-stack confocal images were projected in the y-z plane. Arrowheads indicate the position of MCC apical surface. Scale bars, 3 µm. (C) Basal body docking defect at the MCC apical region upon Drg1 knockdown. CLAMP-GFP (green) and membrane-RFP (red) mRNAs were injected with the indicated morpholinos and mRNAs. Top panels indicate apical region of MCCs and bottom panels 2 µm below the apical region. Scale bars, 5 µm.