Manipulations of endosomal trafficking alter wild type dSNX16 endosome structure, localization and distribution in the neuron. (A) Representative images of animals expressing VGlut-GAL4-driven UAS-dSnx16-GFP together with Rab5^CA, or Rab7^CA at the NMJ, the proximal axon, and the cell body. myr-mRFP serves as a UAS control. (B) dSNX16-GFP mean intensity quantification at the NMJ and the cell body. (C) CoV of dSNX16-GFP at proximal axon. (D) Representative SIM images of animals expressing VGlut-GAL4-driven UAS-dSnx16-GFP together with Rab5^DN or Rab5^CA at the cell body. L, left panel; R, right panel. (E) Representative SIM images of animals expressing VGlut-GAL4–driven UAS-dSnx16-SNAP variants together with Rab5^CA in the dSnx16 null background. Quantification is from ≥23 NMJs, 35 axons, or 57 cell bodies analyzed using a Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Intensity measurements were normalized to mean intensity in the wild-type dSNX16 condition. All images show 2D maximum intensity projections of confocal stacks unless noted otherwise. Data are shown as box-and-whisker plots with all data points superimposed. **, P < 0.01; ***, P < 0.001. ns, not significant. Scale bars, 10 µm.