The dSNX16 CC domain regulates its endosomal localization specifically in the cell body. (A and B) Representative images of animals expressing VGlut-GAL4–driven dSnx16-SNAP transgenes. (C) PCCs between dSNX16 and Rab5, Rab7, α-Rab11 immunoreactivity, or UAS-GFP-myc-2xFYVE at the NMJ. (D) PCC between dSNX16 and endogenous GFP-Rab5, YFP-Rab7, α-Rab11 immunoreactivity, or UAS-GFP-myc-2xFYVE in the cell body. (E) Mean intensity quantification of Rab5, Rab7, and FYVE at the NMJ. (F) Mean intensity quantification of Rab5, Rab7, and FYVE in the cell body. Quantification is from ≥21 NMJs or 52 cell bodies, analyzed using a Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Intensity measurements were normalized to mean intensity in the wild-type dSNX16 condition. All images show 2D maximum intensity projections of confocal stacks. Data are shown as box-and-whisker plots with all data points superimposed. *, P < 0.05; **, P < 0.01; ***, P < 0.001. ns, not significant. Scale bars, 10 µm.