![Figure 5. dSNX16 CC variants alter endosome structure, localization, and distribution in larval motor neurons. (A and B) Representative images of animals expressing indicated UAS-dSnx16-SNAP variants driven by VGlut-GAL4. Shown are muscle 4 NMJ, proximal axons (within 100 µm of the ventral ganglion), and MNISN-I cell bodies (motor neuron in the intersegmental nerve I [Choi et al., 2004a]; see Fig. S2 A for schematics). dSNX16ΔCC reduces and dSNX163A enhances dSNX16 punctate localization. dSNX163A levels are increased at the cell body and reduced at the NMJ. (B) dSNX16 localizes tubular structures at the cell body revealed by SIM. dSNX16ΔCC reduces and dSNX163A increases the quantity of tubulated SNX16 compartments. (C and D) CoV and mean intensity quantification of dSNX16-SNAPJF549. Quantification is from ≥20 NMJs, 42 axons, or 65 cell bodies and analyzed using a Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Intensities were normalized to the mean intensity in the wild-type dSNX16 condition. All images show 2D maximum intensity projections of confocal stacks unless noted otherwise. Data are shown as box-and-whisker plots with all data points superimposed. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Scale bars, 10 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/218/8/10.1083_jcb.201811074/8/jcb_201811074_fig5.jpeg?Expires=1771774885&Signature=LGx1mLK7KhR3JJn67gOgrmzU2aXL5l19b3morkzH3uLC7PFmL~8esKJjdc-n7-Vxwow1SjgY52ioNqlmPWQT8lZEYNlRqxMJya7EbUuTtZi47wuPKtFIN~hKBcmgkB~Yz7kjUWbbaaGEbpFEB9KscoS8i4yPnGNA7i17cELP~adGwj0LUmTTbvlGZxcMRT~2eg~rcVxnz~f0lAj3RUn5QL1knKJ2~VKDa1~tBKFVALb~wuZ6GWKGGWXHaqFwVibzNP3hVKFpFLb2rwqOVC9bHViwYvFvb4O4zhEcArzJLsRRmjngdYF-eBxqsH37O3Uw6K5cyirLRvxMLMdA52F~Ww__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
dSNX16 CC variants alter endosome structure, localization, and distribution in larval motor neurons. (A and B) Representative images of animals expressing indicated UAS-dSnx16-SNAP variants driven by VGlut-GAL4. Shown are muscle 4 NMJ, proximal axons (within 100 µm of the ventral ganglion), and MNISN-I cell bodies (motor neuron in the intersegmental nerve I [Choi et al., 2004a]; see Fig. S2 A for schematics). dSNX16ΔCC reduces and dSNX163A enhances dSNX16 punctate localization. dSNX163A levels are increased at the cell body and reduced at the NMJ. (B) dSNX16 localizes tubular structures at the cell body revealed by SIM. dSNX16ΔCC reduces and dSNX163A increases the quantity of tubulated SNX16 compartments. (C and D) CoV and mean intensity quantification of dSNX16-SNAPJF549. Quantification is from ≥20 NMJs, 42 axons, or 65 cell bodies and analyzed using a Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Intensities were normalized to the mean intensity in the wild-type dSNX16 condition. All images show 2D maximum intensity projections of confocal stacks unless noted otherwise. Data are shown as box-and-whisker plots with all data points superimposed. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Scale bars, 10 µm.
