Figure 3.
![Figure 3. hSNX16 generates membrane tubules via its CC region. (A) Representative single confocal slices of rhodamine PE–labeled GUVs (0%, 1% [30 nM], or 10% [300 nM] PI(3)P) incubated with 100 nM or 500 nM hSNX16 CC variants. Scale bars, 10 µm. (B) Percentage of tubulated vesicles at the indicated PI(3)P and hSNX16 concentrations. Quantification is from at least 20 GUVs per condition. (C) Negative-stain EM of liposomes incubated with buffer, hSNX1, or hSNX16 CC variants (2 µM final protein concentration). Two representative fields are shown for each condition. Scale bar, 400 nm.](https://cdn.rupress.org/rup/content_public/journal/jcb/218/8/10.1083_jcb.201811074/8/jcb_201811074_fig3.jpeg?Expires=1773529181&Signature=UH2WSwPDSFX2DIaEEr7-btwd3vUJeYcLHY4hj~3yg0QYfRqQaQi~uPDtAi3e6f6BQLqslPojtMtV4k-96BtNrZy6ub-tVkxcnALlF-kxksR10E8zeS~uXD969ANBcV4JxVHh7pQHS~rYb0D68WzkYvjWgTapclY7v0lJlaEJW39zukO8FmOWgOBXGrjsn4wW0cQ6-MO2wgnygUNDiraypVMQbtp638vUVAVPfOjG1ZAdIH7A6eCFLT5eokMSiXnR7pUiOLTjyRmyzoyew5l3z0~EAr9WWAs7tvDrJaqMfC-iG1sKU3Qzt82QVJiYSSsqcZEiSpvrqzQlVRcAvC~Aog__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
hSNX16 generates membrane tubules via its CC region. (A) Representative single confocal slices of rhodamine PE–labeled GUVs (0%, 1% [30 nM], or 10% [300 nM] PI(3)P) incubated with 100 nM or 500 nM hSNX16 CC variants. Scale bars, 10 µm. (B) Percentage of tubulated vesicles at the indicated PI(3)P and hSNX16 concentrations. Quantification is from at least 20 GUVs per condition. (C) Negative-stain EM of liposomes incubated with buffer, hSNX1, or hSNX16 CC variants (2 µM final protein concentration). Two representative fields are shown for each condition. Scale bar, 400 nm.
