Map-based cloning of the Dek5 gene. (A) Integrated physical-genetic map of the dek5 locus. B73 AGP_v3 physical coordinates are given for molecular markers with recombinants/number of meiotic products genotyped. Bacterial artificial chromosome (BAC) sequences were aligned with blastn to determine overlap regions. Genes in the fine-map interval are denoted with triangles except for dek5, GRMZM2G083374. (B) Dek5 locus schematic. Black boxes are coding exons, gray boxes are untranslated regions, and introns are lines. Triangles indicate Mu insertions with dek5-PS25 having a Mu8-like insertion at the same site as dek5-MS33 with a Mu1 insertion. (C) Complementation test of dek5-PS25 with UniformMu allele, mu1021358 (dek5-umu1). (D) Complementation test of dek5-PS25 with UniformMu allele, mu1047944 (dek5-umu2). Arrows indicate noncomplementing mutants in a single row of kernels. (E) DEK5 protein domains: N-terminal TP, a single-pass α-helical TMD, and a C-terminal TamB domain. Brackets indicate regions for the C-terminal GFP fusion (DEK5N-GFP) and antibody production (α-DEK5).