dek5 kernel phenotypes. (A) Sagittal sections of mature kernels for normal (B73) and dek5 alleles in B73. (B) Abgerminal view of mature kernels for normal, three alleles of dek5, and bt1, bt2, and sh2 mutants. Scale bars are 2 mm. (C) Proportional pie charts of average mature composition and weight for 20 kernels of dek5-MS33 and normal siblings. Percent oil is black; protein is orange; starch is blue; other constituents are white. (D) Mean and standard deviation of endosperm starch for dek5 and normal siblings (n = 3 biological replicates). d.w., dry weight. (E) Iodine stained sagittal sections of developing kernels. Starch stains black. Scale bar is 2 mm. (F) Zein levels during development and at maturity (M). Protein was loaded on equal dry tissue weight. (G) Mean and standard deviation of endosperm metabolites (n = 3 biological replicates).