Figure 4.
Figure 4. BAF is required to functionally repair the ruptured NE. (A) NIH3T3 cells expressing GFP-NLS were transfected with siControl, siBAF, or siLaA/C for 96 h before laser-induced NE rupture (purple arrowheads). Bar, 10 µm. (B) The cytoplasmic/nucleoplasmic ratios of GFP-NLS were measured over 15 min after laser-induced NE rupture. The graph represents mean values ± SEM (n = 10 cells for each; **, P < 0.0001 from siControl by a mixed-effects model with Tukey’s post hoc comparison test). (C) Quantification of cytoplasmic/nucleoplasmic ratios of GFP-NLS from NIH3T3 cells transfected with either siControl of siBAF siRNAs before rupture via constricted migration in constrained channels (height, 3 µm). The graph represents mean values ± SEM (n = 10 cells for each; **, P < 0.0001 from siControl by a mixed-effects model).

BAF is required to functionally repair the ruptured NE. (A) NIH3T3 cells expressing GFP-NLS were transfected with siControl, siBAF, or siLaA/C for 96 h before laser-induced NE rupture (purple arrowheads). Bar, 10 µm. (B) The cytoplasmic/nucleoplasmic ratios of GFP-NLS were measured over 15 min after laser-induced NE rupture. The graph represents mean values ± SEM (n = 10 cells for each; **, P < 0.0001 from siControl by a mixed-effects model with Tukey’s post hoc comparison test). (C) Quantification of cytoplasmic/nucleoplasmic ratios of GFP-NLS from NIH3T3 cells transfected with either siControl of siBAF siRNAs before rupture via constricted migration in constrained channels (height, 3 µm). The graph represents mean values ± SEM (n = 10 cells for each; **, P < 0.0001 from siControl by a mixed-effects model).

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