Recruitment of membranes and membrane proteins to nuclear ruptures is impaired by the loss of BAF. (A) NIH3T3 cells expressing GFP-tagged LEM-domain proteins LEMD2, Man1, Emerin, Ankle2, Lap2α, or Lap2β were transfected with siBAF, siControl, or siLaA/C (for GFP-Emerin–expressing cells) for 72 h before laser-induced nuclear rupture (purple arrowheads). Accumulation, or the lack thereof, of each LEM-domain protein at rupture sites (yellow arrowheads) is monitored over 5 min. (B) NIH3T3 cells coexpressing GFP-Chmp7 and cGAS-mCherry were transfected with siBAF, siControl, or siLEMD2 for 72 h before laser-induced nuclear rupture, and GFP-Chmp7 accumulation was monitored over 5 min. Merged channels in zoomed-in images in the bottom row show GFP-Chmp7 (green) and cGAS-mCherry (red). (C) LEMD2-GFP expressing NIH3T3 cells were transfected with either siBAF or siControl siRNAs for 96 h, followed by incubation with ER-tracker red before laser-induced NE rupture. Cells were imaged for 5 min to monitor GFP-LEMD2 and ER-tracker red accumulation at nuclear ruptures (yellow arrowheads). Bars, 5 µm. Yellow boxes indicate the area of zoomed images. Bar, 1 µm.