BAF rapidly but transiently localizes to sites of nuclear rupture. (A) Sequential images of a representative NIH3T3 cell expressing GFP-BAF after nuclear rupture via compression by a blunted microcapillary. GFP-BAF localizes to sites of nuclear rupture on the nuclear rim (yellow arrowheads) and resolves within minutes. Coexpression of mCherry-NLS serves as a nuclear rupture marker (blue arrowheads). (B) Sequential images of a representative NIH3T3 coexpressing GFP-BAF and cGAS-mCherry undergoing laser-induced NE rupture (purple arrowheads). Sites of nuclear rupture are indicated by cGAS accumulation (yellow arrowheads). (C) Sequential images of a representative NIH3T3 cell coexpressing GFP-BAF and mCherry-NLS undergoing nuclear rupture during constricted migration through a channel (height, 3 µm) in a microfluidic device. Sites of nuclear rupture are indicated by BAF accumulation (yellow arrowheads). (D) Populations of MCF10A cells that were compressed, fixed at indicated time points, and labeled with anti-BAF or LEMD2 (green) and HSP90 (red). Hoechst dye was used to label DNA (blue). Cells with ruptured nuclei are apparent at 10 s in BAF-labeled cells and 5 min in LEMD2-labeled cells (yellow arrowheads). Bars: (A–C) 10 µm; (D) 50 µm.