Duf and Pyd depend on each other to localize at SDs. (A–A″) Duf and Pyd staining in pyd attenuated larval nephrocytes; arrows point to Duf/Pyd at SDs and arrowheads to Duf surplus at cell contacts (n = 49N/5S). (B and B′) In duf^pmf1 larval nephrocytes, Duf colocalizes with Pyd at SDs (arrows); excess of Pyd accumulates at external puncta (arrowheads; n = 16N/4S). (C–C″) In duf^sps1 larval nephrocytes, Pyd accumulates at external puncta (arrows) and cell contacts (arrowheads, n = 59N/5S). (D–D″) In stage-16 duf^sps1 nephrocytes, Pyd is located in external aggregates (arrowheads); the square marks the region magnified in D′ and D″ (n = 81N). Scale bars represent 10 µm.