The canonical Pins and Hippo pathways are not required for planar spindle orientation in the wing disc. (A–G) Quantification of mitotic spindle alignment in cells expressing Gli-RNAi (A and B) and (E) hpo-RNAi (nub-Gal4>RNAi), as well as pins^P62 (C), Gαi^P8 (D), hpo^KC202 (F), and wts^X1 (G) MARCM clones. The red and green lines show the median angular deviation for experiments and controls, respectively, and n indicates the number of spindles observed. nub-Gal4 (n = 86; A, B, and E), FRT82B (n = 33; C and G), FRT2A (n = 70; D), FRT42D (n = 46; F) for controls (Fig. S3). MARCM clones were generated using hsFLP UAS-mCD8-GFP; tub-Gal4 FRT82B tub-Gal80/TM6C (C and G), yw hsFLP tub-Gal4 UAS-nlsGFP;; tub-Gal80 FRT2A/TM6B (D), or hsFLP UAS-mCD8-GFP; FRT42D tub-Gal80; tub-Gal4/TM6B (F). n.s., not significant. Scale bars: 5 µm.