Figure 8.
Figure 8. PI4KIIIβ is a positive regulator of starvation-induced autophagy. (A) HEK293A cells were treated with RF or PI4KIIIβ siRNA for 72 h and then incubated in FM or ES without or with Bafilomycin A1 (EB) for 2 h and immunoblotted for PI4KIIIβ, actin, and LC3B. (B) Quantification of A; statistical analysis using one-way ANOVA with Tukey’s multiple comparisons test, mean ± SEM, n = 7 experiments; *, P ≤ 0.05; **, P ≤ 0.01. (C) HEK293A cells were treated with RF or PI4KIIIβ siRNA for 72 h, incubated in ES for 2 h, fixed, and labeled using antibodies to WIPI2 or LC3. Scale bars, 10 µm. (D) WIPI2 and LC3B puncta in cells treated as in C were counted. Statistical analysis using two-tailed unpaired Student’s t test, mean ± SEM, n = 3 experiments, 100 cells per condition per independent experiment were quantified; ***, P ≤ 0.001.

PI4KIIIβ is a positive regulator of starvation-induced autophagy. (A) HEK293A cells were treated with RF or PI4KIIIβ siRNA for 72 h and then incubated in FM or ES without or with Bafilomycin A1 (EB) for 2 h and immunoblotted for PI4KIIIβ, actin, and LC3B. (B) Quantification of A; statistical analysis using one-way ANOVA with Tukey’s multiple comparisons test, mean ± SEM, n = 7 experiments; *, P ≤ 0.05; **, P ≤ 0.01. (C) HEK293A cells were treated with RF or PI4KIIIβ siRNA for 72 h, incubated in ES for 2 h, fixed, and labeled using antibodies to WIPI2 or LC3. Scale bars, 10 µm. (D) WIPI2 and LC3B puncta in cells treated as in C were counted. Statistical analysis using two-tailed unpaired Student’s t test, mean ± SEM, n = 3 experiments, 100 cells per condition per independent experiment were quantified; ***, P ≤ 0.001.

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