Furin-dependent Bnl cleavage in the wing disc. (A–F′) The αHA-stained (red) wing disc that expressed Bnl:HA1GFP3 under bnl-Gal4 and were ex vivo cultured for 0 (pretreat) to 16 h in the absence and 1–5 h in the presence of Furin inhibitors as indicated. Arrow, truncated Bnl:GFP3 derivative; arrowhead, uncleaved Bnl:HA1GFP3; blue, phalloidin-Alexa Fluor 647 marking cell outlines; merged (A–D) and either split green, red (A′–C″) or only red (D′–F′) channels are shown. (G) Graphs comparing average levels of colocalized HA and GFP in the ASP grown in presence and absence of Furin inhibitors; samples were harvested at different time points from the continuous culture. n = 11 (0 h), 11 (1 h), 10 (2.5 h), 9 (5 h control), 12 (5 h test), 5 (16 h); P values (ANOVA followed by Tukey HSD): P = 0.0001 for 5 h versus either 0 h, 1 h, or 2.5 h of Furin inhibition. Scale bars: 30 µm.
