Cargo sometimes shows a peculiar behavior between the zones within a maturing cisterna. (A) Top panels show enlarged images of cargo (green), cis-Golgi (red), and TGN (blue) markers during the transitional period of cisternal maturation (−85.5 to −38.0 s) in Fig. 6 B. The second to fourth rows in the left show cargo localization within the maturing cisterna. Note the presence of distinct zones labeled with cis-Golgi and TGN markers. Right panels show the transition from cis to TGN zones during maturation, which manifests clear segregation. Scale bar, 250 nm. (B) Graphs show relative fluorescence profiles of cargo (green), cis-Golgi (red), and TGN (blue) markers along the white lines in the maturing cisterna shown above. Note that cargo almost reached the TGN zone at −57.0 s (see arrowheads), but a small peak reappeared in the cis-Golgi zone at −47.5 s (arrow at −47.5 s). Cargo finally accumulated in the TGN zone (arrowhead at −36.0 s). Arrowheads indicate the peak positions of Axl2-GFP fluorescence intensities. Scale bar, 250 nm.