Figure 5.
Figure 5. Cargo moves from cis- to trans-Golgi zones within a cisterna during maturation. (A) Top panels show enlarged images of Axl2-GFP (cargo, green), Mnn9-mCherry (cis-Golgi, red), and Sys1-iRFP (trans-Golgi, blue) during the transitional period of cisternal maturation (−14.4 to 0 s) shown in Fig. 4 B. The second to fourth rows in the left show cargo localization within the maturing cisterna, which harbors distinct zones labeled with cis- and trans-Golgi markers. Right panels show the segregation of cis- and trans-zones in the maturing cisterna. Scale bar, 250 nm. (B) Graphs show relative fluorescence intensities of Axl2-GFP (cargo, green), Mnn9-mCherry (cis, red), and Sys1-iRFP (trans, blue) along the white lines in the maturing cisterna at −9.6 s (left) and −4.8 s (right). Arrowheads indicate the peak positions of Axl2-GFP fluorescence intensities. Scale bar, 250 nm.

Cargo moves from cis- to trans-Golgi zones within a cisterna during maturation. (A) Top panels show enlarged images of Axl2-GFP (cargo, green), Mnn9-mCherry (cis-Golgi, red), and Sys1-iRFP (trans-Golgi, blue) during the transitional period of cisternal maturation (−14.4 to 0 s) shown in Fig. 4 B. The second to fourth rows in the left show cargo localization within the maturing cisterna, which harbors distinct zones labeled with cis- and trans-Golgi markers. Right panels show the segregation of cis- and trans-zones in the maturing cisterna. Scale bar, 250 nm. (B) Graphs show relative fluorescence intensities of Axl2-GFP (cargo, green), Mnn9-mCherry (cis, red), and Sys1-iRFP (trans, blue) along the white lines in the maturing cisterna at −9.6 s (left) and −4.8 s (right). Arrowheads indicate the peak positions of Axl2-GFP fluorescence intensities. Scale bar, 250 nm.

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