Figure 8. CRACR2a is involved in... | Rockefeller University Press

Figure 8.

$Figure 8. CRACR2a is involved in endosomal trafficking of CD47. (A) CD47 but not Transferin (Tfn) is internalized into CRACR2a compartment at the MTOC. Scale bar: 5 µm. Inset scale bar: 2 µm. (B) Quantification of the fraction of internalized receptor that colocalizes with the CRACR2a compartment. Data are from at least 18 cells collected during three independent experiments. Errors bar indicate SEM.(C) Ionomycin stimulates CD47 internalization into CRACR2a compartment. Scale bar: 10 µm. (D) Quantifications of the fraction of internalized CD47 in CRACR2a compartment in EGTA or ionomycin condition. Data are from at least 20 cells collected during three independent experiments. Error bar indicates SEM. **, P < 0.01, Student’s t test. (E) Deletion of CRACR2a reduced the amount of CD47 internalized into MTOC proximal region (<2.5 µm). Scale bar: 5 µm. Data are from at least 149 cells collected during three independent experiments. Error bar indicates SEM. ****, P < 0.0001, Student’s t test.$

CRACR2a is involved in endosomal trafficking of CD47. (A) CD47 but not Transferin (Tfn) is internalized into CRACR2a compartment at the MTOC. Scale bar: 5 µm. Inset scale bar: 2 µm. (B) Quantification of the fraction of internalized receptor that colocalizes with the CRACR2a compartment. Data are from at least 18 cells collected during three independent experiments. Errors bar indicate SEM.(C) Ionomycin stimulates CD47 internalization into CRACR2a compartment. Scale bar: 10 µm. (D) Quantifications of the fraction of internalized CD47 in CRACR2a compartment in EGTA or ionomycin condition. Data are from at least 20 cells collected during three independent experiments. Error bar indicates SEM. **, P < 0.01, Student’s t test. (E) Deletion of CRACR2a reduced the amount of CD47 internalized into MTOC proximal region (<2.5 µm). Scale bar: 5 µm. Data are from at least 149 cells collected during three independent experiments. Error bar indicates SEM. ****, P < 0.0001, Student’s t test.