Figure 4.
Figure 4. T cell activation promotes the formation of CRACR2a puncta at the cell cortex. (A) Formation of CRACR2a cortical puncta at the periphery of the IS. Scale bar: 5 µm. (B) Quantification of the number of observed CRACR2a cortical puncta when Jurkat cells are stimulated by different conditions. Control, IgG isotype control; Iono, stimulation with ionomycin/calcium. n = 29–41 ROIs from at least 20 cells collected during three independent experiments. Error bar indicates SEM. ****, P < 0.0001, one-way ANOVA with Tukey’s multiple comparisons test.

T cell activation promotes the formation of CRACR2a puncta at the cell cortex. (A) Formation of CRACR2a cortical puncta at the periphery of the IS. Scale bar: 5 µm. (B) Quantification of the number of observed CRACR2a cortical puncta when Jurkat cells are stimulated by different conditions. Control, IgG isotype control; Iono, stimulation with ionomycin/calcium. n = 29–41 ROIs from at least 20 cells collected during three independent experiments. Error bar indicates SEM. ****, P < 0.0001, one-way ANOVA with Tukey’s multiple comparisons test.

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