Figure 2.
Figure 2. ATR signaling in ETAA1 and TOPBP1-deficient cell lines. (A) HCT116 (WT), ETAA1ΔAAD, TOPBP1-AID, and ETAA1ΔAAD/TOPBP1-AID cells were treated with DMSO or IAA for 2 or 24 h and γH2AX induction measured by immunofluorescence imaging. Representative images and quantification of two independent experiments are show. Displayed is mean and SEM for each sample with >1,000 cells measured for each condition. Bar, 10 µm. (B) Cells were treated with 100 nM CPT for 4 h following a 2-h pretreatment with IAA or DMSO as indicated, then lysed and immunoblotted with the indicated antibodies. Molecular weight is given in kilodaltons.

ATR signaling in ETAA1 and TOPBP1-deficient cell lines. (A) HCT116 (WT), ETAA1ΔAAD, TOPBP1-AID, and ETAA1ΔAAD/TOPBP1-AID cells were treated with DMSO or IAA for 2 or 24 h and γH2AX induction measured by immunofluorescence imaging. Representative images and quantification of two independent experiments are show. Displayed is mean and SEM for each sample with >1,000 cells measured for each condition. Bar, 10 µm. (B) Cells were treated with 100 nM CPT for 4 h following a 2-h pretreatment with IAA or DMSO as indicated, then lysed and immunoblotted with the indicated antibodies. Molecular weight is given in kilodaltons.

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