Figure 1.
MPS1 and CCNB1 localize to unattached kinetochores. (A) HeLa CCNB1-GFP/MPS1-mCherry cells were stained with CREST serum to label kinetochores. Representative images of cells at different phases of mitosis and after 5-min treatment with 3 µM nocodazole (right) are shown. (B) HeLa CCNB1-GFP cells at the different stages of mitosis were stained with antibodies for Astrin and kinetochores (CREST).