Figure 3.
Figure 3. MPS1 is a target of the B55-regulated exit pathway. (A) Unified wiring diagram for the spindle checkpoint and PP2A-B55 pathway. (B–D) Simulation of the system described in the wiring diagram was performed for control (siControl; B), 80% PP2A-B55–depleted (siB55 0.2; C), and 90% MASTL-depleted (siMASTL 0.1; D) conditions. Experimental data points obtained by mass spectrometry are marked by an X, while simulated output is shown in solid lines. (E) Simulation of checkpoint response under conditions used for the checkpoint response assays in Fig. 1 (B and D). (F) Mitotically phosphorylated MPS1-GFP purified from 20 mg of cell lysate was incubated with purified PP2A-B55 or buffer control, and dephosphorylation was analyzed by mass spectrometry. The intensities of the phospho-peptides covering S281 and S821 were plotted.

MPS1 is a target of the B55-regulated exit pathway. (A) Unified wiring diagram for the spindle checkpoint and PP2A-B55 pathway. (B–D) Simulation of the system described in the wiring diagram was performed for control (siControl; B), 80% PP2A-B55–depleted (siB55 0.2; C), and 90% MASTL-depleted (siMASTL 0.1; D) conditions. Experimental data points obtained by mass spectrometry are marked by an X, while simulated output is shown in solid lines. (E) Simulation of checkpoint response under conditions used for the checkpoint response assays in Fig. 1 (B and D). (F) Mitotically phosphorylated MPS1-GFP purified from 20 mg of cell lysate was incubated with purified PP2A-B55 or buffer control, and dephosphorylation was analyzed by mass spectrometry. The intensities of the phospho-peptides covering S281 and S821 were plotted.

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