Figure 2.
Figure 2. Extended checkpoint responsiveness in the absence of PP2A-B55. (A) Control (siControl) and PP2A-B55–depleted (siB55) Hela GFP-MAD2 cells, left untreated or exposed to 8.25 nM nocodazole, were imaged every 2 min passing through mitosis. Images show the behavior of MAD2 in the presence of 8.25 nM nocodazole with two examples of the siB55 condition. (B) Cumulative mitotic exit frequency was measured in the absence (siCon; n = 28) or presence of 8.25 nM nocodazole (siCon +Noc; n = 76) and following PP2A-B55 depletion in the absence (siB55; n = 33) or presence of 8.25 nM nocodazole (siB55 +Noc; n = 66). (C) NEBD-anaphase duration in 8.25 nM nocodazole is plotted; each point represents an individual cell with mean NEBD-anaphase time and SD shown as bars. ****, P < 0.0001 (Student’s t test).

Extended checkpoint responsiveness in the absence of PP2A-B55. (A) Control (siControl) and PP2A-B55–depleted (siB55) Hela GFP-MAD2 cells, left untreated or exposed to 8.25 nM nocodazole, were imaged every 2 min passing through mitosis. Images show the behavior of MAD2 in the presence of 8.25 nM nocodazole with two examples of the siB55 condition. (B) Cumulative mitotic exit frequency was measured in the absence (siCon; n = 28) or presence of 8.25 nM nocodazole (siCon +Noc; n = 76) and following PP2A-B55 depletion in the absence (siB55; n = 33) or presence of 8.25 nM nocodazole (siB55 +Noc; n = 66). (C) NEBD-anaphase duration in 8.25 nM nocodazole is plotted; each point represents an individual cell with mean NEBD-anaphase time and SD shown as bars. ****, P < 0.0001 (Student’s t test).

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