A p53-dependent mechanism limits the division of micronucleated KIF18A KO cells. (A) Still frames from time-lapse analyses of dividing histone 2B-GFP (H2B-GFP) expressing KIF18A KO cells and KIF18A KO cells treated with p53 siRNAs. (B) Plot of the percent of micronucleated cells that enter mitosis in control, p53 KD, KIF18A KO, or KIF18A KO + p53 KD hTERT-RPE1 cells. (C) Plot of body weights measured at the indicated dpp for each genotype listed. Error bars indicate SD. (D) Survival plot for mice of the indicated genotypes as a function of dpp. (E) Model for abnormal nuclear formation in the absence of chromosome alignment (see Discussion section for details). Data for all analyses were collected from three independent experiments.