Loss of KIF18A function and chromosome alignment disrupts interchromosomal compaction during anaphase and Lamin A/C distribution during telophase. (A) Representative images of anaphase cells fixed and stained for α-tubulin (red) and centromeres (ACA; green). (B and C) Histograms of centromere to pole distance variance (calculated as SD) among all centromeres within a half spindle of control and KIF18A siRNA-treated hTERT-RPE1 cells (B) or control and KIF18A KO hTERT-RPE1 cells (C). Data were compared using a Kolmogorov–Smirnov t test; P < 0.01. (D) Representative images of control and KIF18A KO telophase cells labeled with Lamin A/C antibodies. (E) Plots of Lamin A/C fluorescence profiles along the long axis of telophase nuclei from control and KIF18A KO hTERT-RPE1 cells. (F) Box and whisker plot of Lamin A/C fluorescence variance in telophase nuclei calculated as the DAI in control and KIF18A KO cells. Bars indicate mean and SD. Distributions of DAI were compared using a Kolmogorov–Smirnov t test; P < 0.005. (G) Percentage of telophase cells with a DAI >2 SDs above the average control DAI. Data were compared using a χ2 test. *, P < 0.005 All data were collected from three independent experiments.
