MN and abnormal nuclear shapes form as KIF18A-deficient cells exit mitosis. (A) Representative stills from time-lapse images of control and KIF18A KO hTERT-RPE1 cells expressing histone H2B-GFP. Note that MN (arrow, middle row) and lobed primary nuclei (bottom row) form as KIF18A KO cells exit mitosis. (B) Plot of the percentage of daughter cells that form MN during mitotic exit. Data were compared via χ² test; **, P < 0.01. (C) Quantification of mother cell nuclear solidity measured 60 min before metaphase. Bars indicate mean and SD. Data were compared via Kolmogorov–Smirnov t test; P > 0.90. (D) Percentage of mother cell nuclear solidity values less than two SDs from the average control solidity. Data were compared via χ² test; P > 0.90. (E) Quantification of daughter cell nuclear solidity 20 min after initial chromatin decondensation. Bars indicate mean and SD. Data were compared via Kolmogorov–Smirnov t test; *, P < 0.05. (F) Percentage of daughter cell nuclear solidity values two SDs below the average control solidity. Data were compared using χ² tests; **, P < 0.01. All data were collected from three independent experiments.