Altered KBP expression affects kinetochore oscillatory movements. (A) Example traces showing the movement over time of representative kinetochore pairs relative to the metaphase plate (dotted line) in cells from the indicated experimental conditions. (B) Representative kymographs of GFP-tagged CENP-B dynamics in metaphase cells transfected with the indicated siRNAs or plasmids. Full videos (Videos 5, 6, 7, and 8) are available in the online supplemental material. (C) Quantification of the DMP, DAP, and mCherry (mCh) fluorescence. DMP and DAP values were normalized to control siRNA or mCh-only conditions. *, adjusted P < 0.05; ****, adjusted P < 0.0001 with 95% confidence interval by one-way ANOVA with Tukey’s multiple comparisons test. Error bars represent SD. Data were obtained from the following numbers of cells over two or three independent experiments: control siRNA (10 [97 kinetochores]), KBP siRNA (12 [83 kinetochores]), mCh only (8 [69 kinetochores]), and mCh-KBP (7 [75 kinetochores]). OE, overexpression.