Figure 7. YAP and TAZ modulate actin... | Rockefeller University Press

Figure 7.

$Figure 7. YAP and TAZ modulate actin polymerization through NUAK2 regulation. (A) ECFCs were screened for YAP/TAZ-regulated cytoskeletal regulators NUAK2, ARHGAP28, and ARHGAP29; n = 6; *, P < 0.0001, two-tailed Student’s unpaired t test. (B) Cyr61 and NUAK2 expression in migrating ECFCs. n = 3; *, P < 0.0004 versus control at 1 h; #, P < 0.0001 versus 0 h, two-way ANOVA with Tukey’s post hoc test. (C) Fluorescent intensity line profiles of phalloidin, three cells per condition. (D) F- and G-actin visualized with Alexa Fluor 488 secondary and 594 phalloidin. (E) F-actin (P < 0.0001) and G-actin intensity (P < 0.007) and F-/G-actin ratio per cell, normalized to siControl. n = 60 cells; P < 0.005; ANOVA with Tukey’s post hoc test. (F) Western blot of β-actin in F- and G-fractions of ECFC lysate (G) F-/G-actin ratio of β-actin densitometry measurements in F- and G-fractions. n = 4 samples; P > 0.08; ANOVA with Tukey’s post hoc test. n.s., not significant. Repeated significance indicator letters (e.g., a–a) signify P > 0.05, while groups with distinct indicators (a vs. b) signify P < 0.05.$

YAP and TAZ modulate actin polymerization through NUAK2 regulation. (A) ECFCs were screened for YAP/TAZ-regulated cytoskeletal regulators NUAK2, ARHGAP28, and ARHGAP29; n = 6; *, P < 0.0001, two-tailed Student’s unpaired t test. (B) Cyr61 and NUAK2 expression in migrating ECFCs. n = 3; *, P < 0.0004 versus control at 1 h; #, P < 0.0001 versus 0 h, two-way ANOVA with Tukey’s post hoc test. (C) Fluorescent intensity line profiles of phalloidin, three cells per condition. (D) F- and G-actin visualized with Alexa Fluor 488 secondary and 594 phalloidin. (E) F-actin (P < 0.0001) and G-actin intensity (P < 0.007) and F-/G-actin ratio per cell, normalized to siControl. n = 60 cells; P < 0.005; ANOVA with Tukey’s post hoc test. (F) Western blot of β-actin in F- and G-fractions of ECFC lysate (G) F-/G-actin ratio of β-actin densitometry measurements in F- and G-fractions. n = 4 samples; P > 0.08; ANOVA with Tukey’s post hoc test. n.s., not significant. Repeated significance indicator letters (e.g., a–a) signify P > 0.05, while groups with distinct indicators (a vs. b) signify P < 0.05.