YAP and TAZ modulate FA remodeling, but not integrin β1 recycling, through myosin. (A) Vinculin and F-actin, visualized with Alexa Fluor 594 secondary and 488 phalloidin, respectively. (B) Average number of FAs per cell as a function of cell area. n = 30–34; P < 0.004; ANOVA with Tukey’s post hoc test. (C and D) FA size histogram (C) and cumulative distribution function (D) or the percentage of FAs in a cell of a given size. (E) β1-integrin internalization and recycling. Live ECFCs were incubated with antibodies targeting active β1-integrin (10 µg/ml), which were endocytosed for 45 min, followed by acid wash and fixation. Internalized integrin was detected with Alexa Fluor 488 secondary. RAB7⁺ endosomes were visualized with Alexa Fluor 594 secondary. (F) Total fluorescent intensity of endocytosed β1-integrin. n = 30; P < 0.0001; two-tailed Student’s unpaired t test. (G) Fluorescent intensity of recycling endocytosed β1-integrin colocalized with RAB7⁺ endosomes. (H) MLC and pMLC visualized by Alexa Fluor 594 and 488 secondaries, respectively. (I) Total MLC intensity per cell. n = 25; P > 0.15; two-tailed unpaired Student’s t test. (J) Percentage of MLC phosphorylated at Ser19 (i.e., pMLC/total MLC intensity × 100, per cell). n = 25; P < 0.0001; two-tailed Student’s unpaired t test. (K) Wound closure rates after treatment with Y-27632 (10 µM) or Blebbistatin (20 µM). n = 16; P < 0.0001; ANOVA with Tukey’s post hoc test. n.s., not significant. Repeated significance indicator letters (e.g., a–a) signify P > 0.05, while groups with distinct indicators (a vs. b; *) signify P < 0.05.