YAP and TAZ are dispensable for microtubule polarization. (A) Microtubules (α-tubulin) and Golgi (GM130) were visualized with Alexa Fluor 488 and 594 secondary, respectively. Golgi were considered polarized when within a 120° region centered about a vector extending horizontally from the nuclei (DAPI) in the direction of the wound edge. (B) Schematic of Golgi polarization measurement, where blue circles are nuclei, and Xs are Golgi. (C and D) Rose plot of Golgi polarization in siControl (black) and siYAP/TAZ (purple) in leading (C) and trailing (D) cells. n = 64–65 leading cells; n = 143–165 trailing cells. (E) Percentage of Golgi polarized to the wound in leading and trailing regions; P < 0.02; *, P < 0.002; χ2 test with Bonferroni post hoc test. (F) α-Tubulin and nuclei visualized by Alexa Fluor 488 secondary and DAPI, respectively. Repeated significance indicator letters (e.g., a–a) signify P > 0.05, while groups with distinct indicators (a vs. b) signify P < 0.05.
