YAP and TAZ promote collective and individual cell motility. ECFCs expressing EGFP (siControl) or mTomato (siYAP/TAZ) monolayers were imaged in 15-min intervals over 10 h to track individual cell migration. (A) Representative cell migration tracks over 10 h (dashed line indicates starting position). (B) Instantaneous average cell migration speed (30 min intervals shown) as a function of time. Cells were grouped into leading (< 100 µm from the front most cell) and trailing (100–500 µm from the front most cell) based on their initial position. n = 140–165 leading cells and n = 437–484 trailing cells. (C) Average migration speed per cell over 10 h. P < 0.0001; ANOVA with Tukey’s post hoc test. (D) Schematic of instantaneous cell directionality, defined as the direction, φ(t_n − t_n-1), a cell moved between the current position, t_n, and the previous position, t_n-1, relative to the wound edge. (E) Instantaneous cell directionality in leading and trailing cells. Data points >0° indicate movement toward the wound, whereas those <0° indicate movement away from the wound. (F) Motile persistence, or end-to-end displacement divided by total displacement. P < 0.0001; ANOVA with Tukey’s post hoc test. (G) Random migration of mTomato siYAP/TAZ cells mixed with a 100-fold excess of EGFP siControl cells. (H) Mean square displacement of randomly migrating siControl (R² = 0.57) and siYAP/TAZ (R² = 0.35) cells. (I) Net displacement of randomly migrating cells over 10 h. n = 10; P < 0.0004; two-tailed Student’s unpaired t test. Repeated significance indicator letters (e.g., a–a) signify P > 0.05, while groups with distinct indicators (a vs. b; *) signify P < 0.05.