YAP and TAZ are essential for ECFC motility by limiting cytoskeletal prestress. (A) Average cell density (y = −1863e^−.00177x + 1643) and area (y = 7062 − 6287[1 − e^−.00267x]) as a function of distance from the leading edge (dotted lines) in 100-µm ROIs. (B) TAZ localization visualized by Alexa Fluor 594 secondary and DAPI subdivided into 100-µm ROIs. (C) Normalized total YAP (y = .255e^−.00996 + 1 and TAZ (y = .486e^−.00582x + 1) fluorescent intensity. (D) Normalized nuclear YAP (y = .003e^−.00839x + 1) and TAZ (y = .120e^−.00529x + 1) fluorescent intensity. n = 7; *, P < 0.0001 versus all other ROIs; two-way ANOVA with Tukey’s post hoc test. (E) Confirmation of YAP and TAZ depletion by Western blot. (F) ECFC migration; actin visualized by Alexa Fluor 488 phalloidin. (G) Wound closure rate. n = 16–20; P < 0.0001; ANOVA with Tukey’s post hoc test. (H) Conditioned medium from siControl or siYAP/TAZ was transferred to adjacent wells with siControl or siYAP/TAZ cells. (I) Wound closure after conditioned media treatment. n = 16; P < 0.0001; ANOVA with Tukey’s post hoc test. (J) Apical cell modulus measured by nanoindentation at 100-µm ROIs. n = 10–32 cells per ROI; *, P < 0.023 versus control leading edge; #, P < 0.001 versus siYAP/TAZ monolayer; two-way ANOVA with Tukey’s post hoc test.