Comparative analysis of mitotic histone phosphorylations. (A) RPE-1 cells were stained for H2B S6ph and H3 S10ph in various phases of mitosis with specific antibodies; DNA was revealed by Hoechst dye. Different mitotic phases are shown. Bars, 2 µm. (B) RPE-1 cells were stained for H2B S6ph, H3 T3ph, and the kinetochore marker protein CENP-C during various mitotic phases. Bars, 2 µm. (C) Chromosome spreads were produced from nocodazole-arrested cells, which were stained for phosphorylation of H2B S6 and H3 S10. Bars, 1 µm. (D) RPE-1 cells were treated for 2 h with 2 µM nocodazole, and chromosome spreads were produced to stain for H2B S6ph and H3 T3ph. Bars, 1 µm.